Recombinant Human UBE2I/Ubc9 Protein

Product Discontinued

Recombinant Human UBE2I/Ubc9 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-158 of Human UBE2I/Ubc9
  
  

  Source: E.coli

  Amino Acid Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2I
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 mM HEPES buffer (pH7.5) 150 mM NaCl, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UBE2I/Ubc9 Protein

• C358B7.1
• EC 6.3.2
• EC 6.3.2.-
• p18
• SUMO-1-protein ligase
• SUMO-protein Ligase
• Ubc9
• UBC9SUMO-conjugating enzyme UBC9
• UBCE9
• UBE2I
• Ubiquitin carrier protein 9
• Ubiquitin carrier protein I
• ubiquitin conjugating enzyme 9
• Ubiquitin-conjugating enzyme E2 I
• ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• ubiquitin-conjugating enzyme UbcE2A
• ubiquitin-like protein SUMO-1 conjugating enzyme
• ubiquitin-protein ligase E2I
• Ubiquitin-protein ligase I

Background

Human Ubc9 is homologous to ubiquitin-conjugating enzymes(E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating(E1) and -conjugating(E2) enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2I