Recombinant Human UBE2I/Ubc9 Protein Summary
Description |
A biologically active protein to UBE2I/Ubc9 with an N-terminal His-Tag.
Source: E. coli
Amino Acid Sequence: MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKFPIDY PYSPPAFRFL TKMWHPNIYE TGDVCISILH PPVDDPQSGE LPSERWNPTQ NVRTILLSVI SLLNEPNTFS PANVDASVMY RKWKESKGKD REYTDIIRKQ VLGTKVDAER DGVKVPTTLA EYCVKTKAPA PDEGSDLFYD DYYEDGEVEE EADSCFGDDE DDSGTEES |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
UBE2I |
Purity |
>95% pure by SDS-PAGE |
Endotoxin Note |
Less than 1EU/ug of endotoxin as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
Preservative |
5% Trehalose |
Purity |
>95% pure by SDS-PAGE |
Reconstitution Instructions |
Please contact technical support for detailed reconstitution instructions. |
Alternate Names for Recombinant Human UBE2I/Ubc9 Protein
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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