Recombinant Human UBE2I/Ubc9 Protein

• Reactivity: Hu
• Applications: Func, PAGE
• Format: Carrier-Free

Recombinant Human UBE2I/Ubc9 Protein Summary

• Description: A biologically active protein to UBE2I/Ubc9 with an N-terminal His-Tag.
  
  Source: E. coli
  
  Amino Acid Sequence: MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKFPIDY PYSPPAFRFL TKMWHPNIYE TGDVCISILH PPVDDPQSGE LPSERWNPTQ NVRTILLSVI SLLNEPNTFS PANVDASVMY RKWKESKGKD REYTDIIRKQ VLGTKVDAER DGVKVPTTLA EYCVKTKAPA PDEGSDLFYD DYYEDGEVEE EADSCFGDDE DDSGTEES
• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2I
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 1EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

• Theoretical MW: 28.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human UBE2I/Ubc9 Protein

• C358B7.1
• EC 6.3.2
• EC 6.3.2.-
• p18
• SUMO-1-protein ligase
• SUMO-protein Ligase
• Ubc9
• UBC9SUMO-conjugating enzyme UBC9
• UBCE9
• UBE2I
• Ubiquitin carrier protein 9
• Ubiquitin carrier protein I
• ubiquitin conjugating enzyme 9
• Ubiquitin-conjugating enzyme E2 I
• ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• ubiquitin-conjugating enzyme UbcE2A
• ubiquitin-like protein SUMO-1 conjugating enzyme
• ubiquitin-protein ligase E2I
• Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2I
• Entrez:
  • 7329(Human)
  • 22196(Mouse)
• Uniprot:
  • P49427(Human)