Recombinant Human UBE2I/Ubc9 Protein

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SDS-Page: Human UBE2I/Ubc9 His (N-Term) Protein [NBP2-35094]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human UBE2I/Ubc9 Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 158 residues of UBE2I with an N-terminal His-Tag.

Source: E. coli

Amino Acid Sequence: MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
UBE2I
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human UBE2I/Ubc9 Protein

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I

Background

Human Ubiquitin Conjugating Enzyme E2 I (UBE2I)/SUMO-conjugating enzyme UBC9 (UBC9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. UBC9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I kappa B alpha, and PML without the requirement of an E3 ligase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol UBE2I
Entrez