UBE2I/Ubc9 Antibody (1B10)

Western Blot: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.

Flow Cytometry: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).

Western Blot: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)

Immunohistochemistry: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.

ELISA: UBE2I/Ubc9 Antibody (1B10) [NBP2-37547] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

UBE2I/Ubc9 Antibody (1B10) Summary

• Immunogen: Purified recombinant fragment of human UBE2I expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2I
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for UBE2I/Ubc9 Antibody (1B10)

• C358B7.1
• EC 6.3.2
• EC 6.3.2.-
• p18
• SUMO-1-protein ligase
• SUMO-protein Ligase
• Ubc9
• UBC9SUMO-conjugating enzyme UBC9
• UBCE9
• UBE2I
• Ubiquitin carrier protein 9
• Ubiquitin carrier protein I
• ubiquitin conjugating enzyme 9
• Ubiquitin-conjugating enzyme E2 I
• ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• ubiquitin-conjugating enzyme UbcE2A
• ubiquitin-like protein SUMO-1 conjugating enzyme
• ubiquitin-protein ligase E2I
• Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2I