Recombinant Human UbcH5b/UBE2D2 Protein

Product Discontinued

Recombinant Human UbcH5b/UBE2D2 Protein Summary

• Description: Recombinant protein for Human UbcH5b/UBE2D2
  
  

  Source:E. coli
  Amino Acid Sequence:(P62837)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2D2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH5b/UBE2D2 Protein

• E2(17)KB2
• EC 6.3.2.19
• PUBC1
• UBC4/5
• UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UBC5B
• UBCH4
• UbcH5b
• UBE2D2
• Ubiquitin carrier protein D2
• ubiquitin-conjugating enzyme E2 D2
• Ubiquitin-conjugating enzyme E2(17)KB 2
• Ubiquitin-conjugating enzyme E2-17 kDa 2
• ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D2

Background

UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100 nM to 1uM depending upon the conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D2