Recombinant Human UbcH5b/UBE2D2 Protein

Product Discontinued

Recombinant Human UbcH5b/UBE2D2 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-147 of Human UbcH5b/UBE2D2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2D2
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UbcH5b/UBE2D2 Protein

• E2(17)KB2
• EC 6.3.2.19
• PUBC1
• UBC4/5
• UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UBC5B
• UBCH4
• UbcH5b
• UBE2D2
• Ubiquitin carrier protein D2
• ubiquitin-conjugating enzyme E2 D2
• Ubiquitin-conjugating enzyme E2(17)KB 2
• Ubiquitin-conjugating enzyme E2-17 kDa 2
• ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D2

Background

UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human UBE2D2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D2