Recombinant Human UbcH2/UBE2H Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human UbcH2/UBE2H Protein Summary

Description
Recombinant protein for Human UbcH2/UBE2H

Source:E. coli
Amino Acid Sequence:(P62259)

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2H
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH2/UBE2H Protein

  • E2-14K
  • E2-20K
  • EC 6.3.2.19
  • UBC8
  • UBC8E2-20K
  • UBCH
  • UbcH2
  • UBE2H
  • Ubiquitin carrier protein H
  • ubiquitin-conjugating enzyme E2 H
  • Ubiquitin-conjugating enzyme E2-20K
  • ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
  • ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
  • Ubiquitin-protein ligase H

Background

UbcH2 is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UbcH2 consists of two functionally independent domains, an N-terminal core domain with ubiquitin conjugating activity, and a C-terminal domain that interacts with substrate proteins. This protein is useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 uM depending upon conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2H
Entrez