Recombinant Human UbcH2/UBE2H Protein

Product Discontinued

Recombinant Human UbcH2/UBE2H Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-183 of Human UbcH2/UBE2H
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2H
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 50 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH2/UBE2H Protein

• E2-14K
• E2-20K
• EC 6.3.2.19
• UBC8
• UBC8E2-20K
• UBCH
• UbcH2
• UBE2H
• Ubiquitin carrier protein H
• ubiquitin-conjugating enzyme E2 H
• Ubiquitin-conjugating enzyme E2-20K
• ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
• ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
• Ubiquitin-protein ligase H

Background

UBE2H (Ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2H is Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human UBE2H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2H