UbcH2/UBE2H Overexpression Lysate


Western Blot: UBE2H Overexpression Lysate (Adult Normal) [NBL1-17531] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2H.
Western Blot: UBE2H Overexpression Lysate (Adult Normal) [NBL1-17531] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for UBE2H.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

UbcH2/UBE2H Overexpression Lysate Summary

Transient overexpression lysate of ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) (UBE2H), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC202516 and based on accession number NM_003344. The protein contains a C-MYC/DDK Tag.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UbcH2/UBE2H Overexpression Lysate

  • E2-14K
  • E2-20K
  • EC
  • UBC8
  • UBC8E2-20K
  • UBCH
  • UbcH2
  • UBE2H
  • Ubiquitin carrier protein H
  • ubiquitin-conjugating enzyme E2 H
  • Ubiquitin-conjugating enzyme E2-20K
  • ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
  • ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
  • Ubiquitin-protein ligase H


The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: EnzAct
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: IHC, WB
Species: Hu
Applications: EnzAct
Species: Hu, Mu
Applications: ICC/IF, WB
Species: Mu, Rt
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
Species: Hu
Applications: EnzAct
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: EnzAct
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: IHC, IHC-P, WB

Publications for UbcH2/UBE2H Lysate (NBL1-17531) (0)

There are no publications for UbcH2/UBE2H Lysate (NBL1-17531).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for UbcH2/UBE2H Lysate (NBL1-17531) (0)

There are no reviews for UbcH2/UBE2H Lysate (NBL1-17531). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UbcH2/UBE2H Lysate (NBL1-17531) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional UbcH2/UBE2H Products

Bioinformatics Tool for UbcH2/UBE2H Lysate (NBL1-17531)

Discover related pathways, diseases and genes to UbcH2/UBE2H Lysate (NBL1-17531). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UbcH2/UBE2H Lysate (NBL1-17531)

Discover more about diseases related to UbcH2/UBE2H Lysate (NBL1-17531).

Pathways for UbcH2/UBE2H Lysate (NBL1-17531)

View related products by pathway.

PTMs for UbcH2/UBE2H Lysate (NBL1-17531)

Learn more about PTMs related to UbcH2/UBE2H Lysate (NBL1-17531).

Research Areas for UbcH2/UBE2H Lysate (NBL1-17531)

Find related products by research area.

Blogs on UbcH2/UBE2H

There are no specific blogs for UbcH2/UBE2H, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our UbcH2/UBE2H Overexpression Lysate and receive a gift card or discount.


Gene Symbol UBE2H