TUFM Antibody - BSA Free

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-32051] - Paraffin-embedded SNU16 xenograft, using antibody at 1:100 dilution.

Western Blot: TUFM Antibody [NBP1-32051] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:2000.

Immunoprecipitation: TUFM Antibody [NBP1-32051] - TUFM antibody [N3C3] immunoprecipitates TUFM protein in IP experiments.IP samples: HepG2 whole cell extractA. 35 ug HepG2 whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of TUFM protein by 4 ug TUFM antibody [N3C3] (NBP1-32051)10 % SDS-PAGEThe immunoprecipitated TUFM protein was detected by TUFM antibody [N3C3] (NBP1-32051) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TUFM Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TUFM Antibody - BSA Free (NBP1-32051) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Mouse (94%), Rat (95%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUFM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TUFM Antibody - BSA Free

• EFTu
• EF-Tu
• EFTUCOXPD4
• EF-TuMT
• elongation factor Tu, mitochondrial
• P43
• Tu translation elongation factor, mitochondrial

Background

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TUFM