TUFM Antibody (HL2193) - Azide and BSA Free

Western Blot: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody [HL2193] (NBP3-25731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Knockdown Validated: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody [HL2193] (NBP3-25731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - TUFM antibody [HL2193] detects TUFM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUFM stained by TUFM antibody [HL2193] (NBP3-25731) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL2193
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TUFM Antibody (HL2193) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TUFM Antibody (HL2193) - Azide and BSA Free (NBP3-25731) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragmemt of human TUFM.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUFM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TUFM Antibody (HL2193) - Azide and BSA Free

• EFTu
• EF-Tu
• EFTUCOXPD4
• EF-TuMT
• elongation factor Tu, mitochondrial
• P43
• Tu translation elongation factor, mitochondrial

Background

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUFM