TUFM Antibody (CL2242) - Azide and BSA Free

Staining in MCF7 cell line with Anti-TUFM monoclonal antibody) .

Staining in HeLa cell line with Anti-TUFM monoclonal antibody) .

Staining in U2OS cell line with Anti-TUFM monoclonal antibody) .

Staining in A431 cell line with Anti-TUFM monoclonal antibody) .

Staining in U251 cell line with Anti-TUFM monoclonal antibody) .

Lane 1: Marker [kDa]Lane 2: Human cell line HeLa cytoplasmic fractionLane 3: Human cell line HeLa membrane fractionLane 4: Human cell line HeLa nuclear fractionLane 5: Human cell line HeLa chromatin fractionLane 6: Human cell line HeLa cytoskeletal fraction

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.

Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate staining in glomerular cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TUFM antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: CL2242
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TUFM Antibody (CL2242) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TUFM Antibody (CL2242) - Azide and BSA Free (NBP2-36751) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P49411, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (95%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUFM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Immunoprecipitation
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunoprecipitation reported in scientific literature (PMID:31801089).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:31801089).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TUFM Antibody (CL2242) - Azide and BSA Free

• EFTu
• EF-Tu
• EFTUCOXPD4
• EF-TuMT
• elongation factor Tu, mitochondrial
• P43
• Tu translation elongation factor, mitochondrial

Background

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUFM