TSFM Antibody (JE64-29)

Western Blot: TSFM Antibody (JE64-29) [NBP3-32294] - Western blot analysis of TSFM on different lysates with Rabbit anti-TSFM antibody (NBP3-32294) at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 ug/Lane Lane 2: Jurkat cell lysate, 10 ug/Lane Lane 3: K562 cell lysate, 10 ug/Lane Lane 4: Mouse liver tissue lysate, 20 ug/Lane Lane 5: Mouse kidney tissue lysate, 20 ug/Lane Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32294) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TSFM Antibody (JE64-29) [NBP3-32294] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TSFM antibody (NBP3-32294) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32294) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: JE64-29
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TSFM Antibody (JE64-29) Summary

• Description: Novus Biologicals Rabbit TSFM Antibody (JE64-29) (NBP3-32294) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human TSFM aa 1-100/325. (Uniprot: P43897)
• Localization: Mitochondrion.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TSFM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TSFM Antibody (JE64-29)

• COXPD3
• EFTS
• EF-Ts
• EFTSMT
• EF-Tsmt
• mitochondrial elongation factor Ts
• mitochondrial
• Ts translation elongation factor, mitochondrial

Background

Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSFM