Tryptase alpha/TPS1 Antibody (2A10-B5)

Western Blot: Tryptase alpha/TPS1 Antibody (2A10-B5) [H00007177-M01] - TPSAB1 monoclonal antibody (M01), clone 2A10-B5. Analysis of TPSAB1 expression in K-562.

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (2A10-B5) [H00007177-M01] - Analysis of monoclonal antibody to TPSAB1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1 ug/ml.

Sandwich ELISA: Tryptase alpha/TPS1 Antibody (2A10-B5) [H00007177-M01] - Detection limit for recombinant GST tagged TPSAB1 is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Tryptase alpha/TPS1 Antibody (2A10-B5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPSAB1 (AAH28059, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP
• Specificity: TPSAB1 - tryptase alpha/beta 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPSAB1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Tryptase alpha/TPS1 Antibody (2A10-B5)

• TPS1
• TPS2
• TPSAB1
• TPSB1
• tryptase alpha/beta 1

Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPSAB1
• Entrez:
  • 7177(Human)
• OMIM:
  • 191080(Human)
• Uniprot:
  • AAH28059(Human)