Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody - Azide Free

Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525] - Western blot of mouse heart homogenate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser23/24 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).

• Reactivity: Hu, Mu, Rt, Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 conjugated to KLH. Accession # P48787
• Modification: p Ser23, p Ser24
• Specificity: Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser23/24. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Predicted Species: Human (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNNI3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody - Azide Free

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and when cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 it causes regulation of Ca2+- stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both serines 23 and 24 is required for the reduction in Ca2+-sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser23/24 phosphorylation is important for enhanced relaxation in response to prolonged activation of protein kinase C by endothelin in intact myocytes, while Thr144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 21954(Mouse)
• Uniprot:
  • P48787(Mouse)