Troponin I Type 3 (cardiac) Antibody (560cc)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IA, IHC, IP, S-ELISA, AP
Clone
560cc
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Troponin I Type 3 (cardiac) Antibody (560cc) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI, native human cTn complex, or human cTnI peptides. a.a.r. 83-93.
Specificity
This is reactive against troponin. No cross-reaction with skeletal muscle TnI.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunoaffinity Purification
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
Application Notes
Cardiac troponin I Immunoassay: Recommended pairs for quantitative sandwich immunoassay (coating - conjugate): NB100-73087 (Coating) - NB100-73086(Conjugate), NB100-73087 (Coating) - NB110-2546 (Conjugate). Antibody combinations for quantitative sandwich Immunoassay: NB110-8458 + NB100-73087 (mixture for coating) - NB120-10239 + NB110-2546 (mixture for detection), NB100-73084 + NB100-73087 (mixture for coating) - NB110-2546+ NB100-73086 (mixture for detection), NB110-8458 + NB100-73087 (mixture for coating) - NB110-2546+ NB110-12915 (mixture for detection), NB120-10239 + NB100-73087 (mixture for coating) - NB110-2546+ NB110-12915 (mixture for detection).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (560cc)

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

FAQs for Troponin I Type 3 (cardiac) Antibody (NB100-73087). (Showing 1 - 1 of 1 FAQ).

  1. Would you please help suggest antibody pairs for detecting Human Creatine Kinase MB,.Myoglobin,.C-reactive protein, and Troponin I, as well as their positive controls? So far, I can only find NB120-8345 and NB600-1291 for Myoglobin. There seems no Creatine Kinase MB antibody available from Novus. I also found two pairs suggested for Troponin I: NB110-2546(Coating)- NB100-73082(Conjugate); NB100-73087(Coating) - NB110-2546(Conjugate). Would you please help confirm? How about CRP? May we also ask if NB810-73233 can be used as the antigen in ELISA of CRP?
    • For Myoglobin The recommended antibodies are NB120-8345 and NB600-1291 as you mentioned. For Troponin I: As you mentioned: NB110-2546(Coating)- NB100-73082(Conjugate) and NB100-73087(Coating) - NB110-2546(Conjugate). We do not have any matched pairs for Creatine KinasE MB. We do not have any match pairs for CRP as we have just not tested these in house in ELISA. The customer could use our CRP antibodies in a direct ELISA. We have not had any one use this as an antigen as of yet however if your customer is willing to test this as innovators they are more than welcome. See full details of our Innovators Reward Program here or contact us at innovators@novusbio.com for more information.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Troponin I Type 3 (cardiac) Antibody (NB100-73087)

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Blogs on Troponin I Type 3 (cardiac).

Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI3
Entrez
Uniprot