Troponin I Type 3 (cardiac) Antibody (560cc)

• Reactivity: Hu
• Applications: WB, ELISA, IA, IHC, IP, S-ELISA, AP
• Clone: 560cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Troponin I Type 3 (cardiac) Antibody (560cc) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human cTn complex.
• Epitope: a.a.r. 83-93
• Specificity: No cross-reaction with skeletal muscle TnI
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunoassay
  • Immunohistochemistry
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
• Application Notes: Recommended pair for sandwich immunoassay (capture-detection): NB110-2546 - NB100-73087, or NB100-73087 - NB100-73086.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (560cc)

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Troponin I Type 3 (cardiac) Antibody (NB100-73087). (Showing 1 - 1 of 1 FAQ).

1. Would you please help suggest antibody pairs for detecting Human Creatine Kinase MB,.Myoglobin,.C-reactive protein, and Troponin I, as well as their positive controls? So far, I can only find NB120-8345 and NB600-1291 for Myoglobin. There seems no Creatine Kinase MB antibody available from Novus. I also found two pairs suggested for Troponin I: NB110-2546(Coating)- NB100-73082(Conjugate); NB100-73087(Coating) - NB110-2546(Conjugate). Would you please help confirm? How about CRP? May we also ask if NB810-73233 can be used as the antigen in ELISA of CRP?
   • For Myoglobin The recommended antibodies are NB120-8345 and NB600-1291 as you mentioned. For Troponin I: As you mentioned: NB110-2546(Coating)- NB100-73082(Conjugate) and NB100-73087(Coating) - NB110-2546(Conjugate). We do not have any matched pairs for Creatine KinasE MB. We do not have any match pairs for CRP as we have just not tested these in house in ELISA. The customer could use our CRP antibodies in a direct ELISA. We have not had any one use this as an antigen as of yet however if your customer is willing to test this as innovators they are more than welcome. See full details of our Innovators Reward Program here or contact us at innovators@novusbio.com for more information.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 7137(Human)
• Uniprot:
  • P19429()