Troponin I Type 3 (cardiac) Antibody (22B11)

Western Blot: Troponin I (cardiac) Antibody (22B11)[NB120-8290]-Momoclonal antibodies 22B11(A), 10B11(B), and 8E10(C) recognizing dephospho- and phospho- forms of human cardiac Tnl.

• Reactivity: Hu, Po, Bv(-), Ca(-), Fe(-), Fi(-), Gt(-), Mu(-), Rb(-), Rt(-)
• Applications: WB, ELISA
• Clone: 22B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Troponin I Type 3 (cardiac) Antibody (22B11) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cTnI.
• Epitope: a.a.r. 20-24
• Specificity: This detects the unphosphorylated form of cardiac troponin I.No cross-reaction with mono or biphosphorylated troponin I.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Control:

  Troponin T type 1 (slow skeletal) Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Pig. Does not cross-react with Mouse, Rat, Cow, Cat, Dog, Fish, Goat or Rabbit. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (22B11)

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Troponin I Type 3 (cardiac) Antibody (NB120-8290). (Showing 1 - 1 of 1 FAQ).

1. Would you be able to share the protocol used to validate NB120-8290 – TnI mAb (22B11) in WB?
   • The western blot image on our site was submitted to us a while ago via a customer review. From the notes I have, they ran their western blot very similar to our general protocol. However, they used 5% nonfat milk with 1% BSA in TBST for blocking and diluents.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 7137(Human)
  • 21955(Mouse)
  • 171409(Rat)