Troponin I Type 3 (cardiac) Antibody (19C7)

Images

 
Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer ...read more
Chromatin Immunoprecipitation: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - Analysis of murine early primitive ectoderm-like cells, staining Cardiac Troponin I.
Immunoassay: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - recognized dephospho-(light blue 19C7) and biphospho-(dark blue 22B11) forms of cTnl.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fe, Fi, Gt, RbSpecies Glossary
Applications WB, ChIP, Flow, IA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Clone
19C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Troponin I Type 3 (cardiac) Antibody (19C7) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cardiac troponin I.
Epitope
41-49 aa
Specificity
Troponin I Type 3 (cardiac)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
Application Notes
Cardiac troponin I Immunoassay: Recommended pairs for quantitative sandwich immunoassay (coating - conjugate): NB100-73085 (coating) - NB110-2546 (Conjugate); best pairs: NB110-2546 (Coating)- NB100-73082 (Conjugate), NB100-73087 (Coating) - NB110-2546 (Conjugate). Antibody combinations for quantitative sandwich immunoassay: NB110-2546+ NB110-8458 (mixture for coating) - NB120-10239 + NB100-73082 (mixture for detection), NB100-73084 + NB100-73087 (mixture for coating) - NB110-2546+ NB100-73086 (mixture for detection), NB110-8458 + NB100-73087 (mixture for coating) - NB110-2546+ NB110-12915 (mixture for detection), NB120-10239 + NB100-73087 (mixture for coating) - NB110-2546+ NB110-12915 (mixture for detection)NB110-2546+ NB120-10239 (mixture for coating) - NB110-2543 (mixture for detection).
Positive Control
Troponin T type 1 (slow skeletal) Lysate (NBL1-17179)
Reviewed Applications
Read 1 Review rated 5
using
NB110-2546 in the following applications:

Publications
Read Publications using
NB110-2546 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (19C7)

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • cTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1troponin I, cardiac
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle

Background

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Troponin I Type 3 (cardiac) Antibody (NB110-2546)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Review for Troponin I Type 3 (cardiac) Antibody (NB110-2546) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-2546:
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Flow
(1)
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Mouse
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Images Ratings Applications Species Date Details
Flow Cytometry Troponin I Type 3 (cardiac) NB110-2546
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reviewed by:
Ivette Leyva
Flow Mouse 10/09/2014
View

Summary

ApplicationFlow Cytometry
Sample Testeddifferentiated ESC cultures
SpeciesMouse
Lot13/05-T21-C7
CommentsCells incubated with secondary Ab only, were used to establish baseline fluorescence of theses cells and to define the cTntpositive region. Data files were acquired and analyzed using the CellQuest pro v.6 software (Becton Dickinson, Erembodegem, Belgium).

Blocking

Blocking Details1 ml of PBS1x/0.5% BSA/0.1% saponin for 5 min

Primary Anitbody

Dilution Ratiomouse anti-mouse cTnt antibody (dilution 1:800, NB110-2546, Novusbio) for 1h at 4°C

Details

Detection NotesFlow cytometry analyses were performed on a BD FACSCalibur instrument (Becton Dickinson, Erembodegem, Belgium) operating at 488n
Fixation Detailsfixed with 0.5% parafolmaldehyde for 20 min at room temperature
Permeabilization Details1 ml cold PBS 1x/0.5% BSA/0.1% saponin for 5 min
Wash Descriptiontwo washing steps of 5 min with 1 ml of PBS1x/0.5% BSA/0.1% saponin

Comments

CommentsCells incubated with secondary Ab only, were used to establish baseline fluorescence of theses cells and to define the cTntpositive region. Data files were acquired and analyzed using the CellQuest pro v.6 software (Becton Dickinson, Erembodegem, Belgium).

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Troponin I Type 3 (cardiac) Antibody (NB110-2546). (Showing 1 - 2 of 2 FAQs).

  1. Would you please help suggest antibody pairs for detecting Human Creatine Kinase MB,.Myoglobin,.C-reactive protein, and Troponin I, as well as their positive controls? So far, I can only find NB120-8345 and NB600-1291 for Myoglobin. There seems no Creatine Kinase MB antibody available from Novus. I also found two pairs suggested for Troponin I: NB110-2546(Coating)- NB100-73082(Conjugate); NB100-73087(Coating) - NB110-2546(Conjugate). Would you please help confirm? How about CRP? May we also ask if NB810-73233 can be used as the antigen in ELISA of CRP?
    • For Myoglobin The recommended antibodies are NB120-8345 and NB600-1291 as you mentioned. For Troponin I: As you mentioned: NB110-2546(Coating)- NB100-73082(Conjugate) and NB100-73087(Coating) - NB110-2546(Conjugate). We do not have any matched pairs for Creatine KinasE MB. We do not have any match pairs for CRP as we have just not tested these in house in ELISA. The customer could use our CRP antibodies in a direct ELISA. We have not had any one use this as an antigen as of yet however if your customer is willing to test this as innovators they are more than welcome. See full details of our Innovators Reward Program here or contact us at innovators@novusbio.com for more information.
  2. Is the antibody cardiac specific? If no, what is the percentage that it is cardiac specific?
    • It is expected to be cardiac specific and to not cross-react with skeletal troponin I.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Troponin I Type 3 (cardiac).

Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

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Recent Reviews

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Ivette Leyva
10/09/2014
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol TNNI3
Entrez
Uniprot