TRMT12 Antibody - BSA Free

Western Blot: TRMT12 Antibody [NBP1-76583] - Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: TRMT12 Antibody - BSA Free [NBP1-76583] - Immunocytochemistry of TRMT12 in K562 cells with TRMT12 antibody at 10 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRMT12 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2. Amino Acid Squence: TQRYREYLQRQKLFDTQHR
• Specificity: TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRMT12
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Western Blot 0.5 ug/ml
• Control Peptide:

  TRMT12 Peptide (NBP1-76583PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TRMT12 Antibody - BSA Free

• Alpha-amino-alpha-carboxypropyl transferase TYW2
• EC 2.1.1
• EC 2.1.1.32
• EC 2.5.1.-
• FLJ20772
• homolog of yeast tRNA methyltransferase
• TRM12
• tRNA methyltranferase 12 homolog (S. cerevisiae)
• tRNA methyltranferase 12 homolog
• tRNA methyltransferase 12 homolog (S. cerevisiae)
• tRNA-yW synthesizing protein 2
• tRNA-yW-synthesizing protein 2
• TYW2tRNA wybutosine-synthesizing protein 2 homolog

Background

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRMT12
• Uniprot:
  • Q53H54(Human)