Human TRAILR1/TNFRSF10A ELISA Kit (Colorimetric)

ELISA: Human TRAILR1/TNFRSF10A ELISA Kit (Colorimetric) [NBP3-14636] - Example of Human TRAILR1/TNFRSF10A Standard Curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Cell lysates, Cell culture supernatant
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 8 pg/ml

Human TRAILR1/TNFRSF10A ELISA Kit (Colorimetric) Summary

• Description: Incubation: 2 hours 30 minutes
• Specificity: Specific for human TRAILR1/TNFRSF10A. No cross reactivity observed with TRAIL, CD117, IL-6R, IL-2R, CD116, TRAIL R2, TRAIL R3, TRAIL R4, CD178 and Granzyme B..
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 8 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 13.87
• Intra-Assay: CV% < 7.57
• Spike Recovery: 81 to 113%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF10A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Human TRAILR1/TNFRSF10A ELISA Kit (Colorimetric)

• APO2
• CD261 antigen
• CD261
• cytotoxic TRAIL receptor
• Death receptor 4
• DR4 TRAIL receptor 1
• DR4
• TNF-related apoptosis inducing ligand receptor 1
• TNF-related apoptosis-inducing ligand receptor 1
• TNFRSF10A
• TRAIL R1
• TRAILR1
• TRAIL-R1
• TRAILR-1
• TRAILR1MGC9365
• tumor necrosis factor receptor superfamily member 10A
• tumor necrosis factor receptor superfamily, member 10a

Background

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TRAILR1/TNFRSF10A ELISA Kit (NBP3-14636). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFRSF10A