![Western Blot: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Western Blot Analysis of U87 cell lysate using TOP1MT Mouse Monoclonal Antibody (TOPT/613).](http://images.novusbio.com/fullsize/TOP1MT-Antibody-TOP1MT-613-Azide-and-BSA-Free-Western-Blot-NBP3-08306-img0004.jpg "Western Blot: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Western Blot Analysis of U87 cell lysate using TOP1MT Mouse Monoclonal Antibody (TOPT/613).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | TOP1MT/613 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07562). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human TOP1MT protein (Uniprot: Q969P6) |
| Localization | Cytoplasmic (Mitochondria) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TOP1MT |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TOP1MT Antibody (NBP3-08306)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TOP1MT |
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Melanoma stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).](http://images.novusbio.com/fullsize/TOP1MT-Antibody-TOP1MT-613-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08306-img0003.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Melanoma stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).")
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Melanoma stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).](http://images.novusbio.com/fullsize/TOP1MT-Antibody-TOP1MT-613-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08306-img0001.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Melanoma stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).")
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Gallbladder stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).](http://images.novusbio.com/fullsize/TOP1MT-Antibody-TOP1MT-613-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08306-img0002.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/613) - Azide and BSA Free [NBP3-08306] - Formalin-fixed, paraffin-embedded human Gallbladder stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/613).")
