TMPRSS4 Antibody

Western Blot: TMPRSS4 Antibody [NBP1-36997] - Analysis of TMPRSS4 in HEK293 lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. The observed molecular weight is represented by the band appearing at 48 kDa.

• Reactivity: Hu, Pm, Pm
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TMPRSS4 Antibody Summary

• Immunogen: TMPRSS4 Antibody is made to a peptide with sequence HCFRKHTDVFNWK corresponding to internal region according to NP_063947.1, NP_001077416.1.
• Specificity: TMPRSS4 Antibody is expected to recognise both reported isoforms (NP_063947.1 and NP_001077416.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMPRSS4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in lysates of HEK293 and A549 (calculated MW of 48.2 kDa band according to NP_063947.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (100%), Gibbon (100%), Marmoset (100%), Monkey (100%)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMPRSS4 Antibody

• CAPH2
• Channel-activating protease 2
• EC 3.4.21
• EC 3.4.21.4
• EC 3.4.21.43
• Membrane-type serine protease 2
• MT-SP2
• TMPRSS3EC 3.4.21.-
• TMPRSS4
• transmembrane protease serine 4
• transmembrane protease, serine 4
• transmembrane serine protease 3
• Type II membrane serine protease

Background

TMPRSS4, previously known as TMPRSS3, is a 437 amino acid type II transmembrane serine protease located on human chromosome 11q23.3 that encodes 5 isoforms containing a complete coding sequence. This serine protease has a predicted molecular weight of 48 kDa with 2 glycosylation sites and the cleaved soluble protease domain has been detected in cell culture media. Functions of TMPRSS4 include embryo development, viral infection, and cancer. TMPRSS4 plays a role in the epithelial-mesenchymal transition (EMT) process and mediates cell invasion, migration, proliferation and metastasis. Overexpression of TMPRSS4 has been reported for multiple solid tumors including pancreatic, ovarian, thyroid, colorectal, lung, breast, cervical, gallbladder, gastric, and liver cancer, and has been associated with poor overall survival and reduced time to tumor progression (1,2).
TMPRSS4 has also been shown to play a role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 or potentially by TMPRSS4 at the cell surface to facilitate viral entry (3).
References

1. L de Aberasturi A, Calvo A. (2015) TMPRSS4: An Emerging Potential Therapeutic Target in Cancer. Br J Cancer. 112(1):4-8. PMID: 25203520

2. Zeng P., Zhang P., Zhou L., Tang M., Shen Y., Jin J., Zhu Y., Chen M. (2016) TMPRSS4 as an emerging potential poor prognostic factor for solid tumors: A systematic review and meta-analysis. Oncotarget. 7:76327-76336. PMID: 27344186

3. Zang F, Castro MFG, McCune BT, Zeng Q, Rothlauf PW, Sonnek NM, Liu Z, Brulois KF, Wang X, Greenberg HB, Diamond MS, Ciorba MA, Whelan SPJ, and Ding S. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes. Sci Immunol. 5(47): eabc3582. PMID: 32404436

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMPRSS4 Antibody (NBP1-36997)(1)

Publication using NBP1-36997

• Dawelbait G, Winter C, Zhang Y et al. Structural templates predict novel protein interactions and targets from pancreas tumour gene expression data. Bioinformatics 2007-07-01 [PMID: 17646287]

Bioinformatics

• Gene Symbol: TMPRSS4
• Entrez:
  • 56649(Human)
• Uniprot:
  • Q9NRS4(Human)