Recombinant Human Thioredoxin-1 Protein

Product Discontinued

Recombinant Human Thioredoxin-1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-105 of Human Thioredoxin
  
  

  Source: E.coli

  Amino Acid Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

• Details of Functionality: Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TXN
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 11.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Thioredoxin-1 Protein

• Thioredoxin1
• Thioredoxin-1
• TRDX;TRX;TRX1;Trx80;TXN
• Trx1
• TXN
• TXN1

Background

Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Thioredoxin Recombinant Protein (NBC1-18541)(1)

Publication using NBC1-18541

• Beyrath J, Pellegrini M, Renkema H et al. KH176 Safeguards Mitochondrial Diseased Cells from Redox Stress-Induced Cell Death by Interacting with the Thioredoxin System/Peroxiredoxin Enzyme Machinery Sci Rep 2018-04-26 [PMID: 29700325] (BA, Human)

Bioinformatics

• Gene Symbol: TXN