Testisin/Prss21 Antibody

Immunocytochemistry/Immunofluorescence: Testisin/Prss21 Antibody [NBP1-84789] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Testisin/Prss21 Antibody [NBP1-84789] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Testisin/Prss21 Antibody [NBP1-84789] - Staining of human endometrium shows no membranous positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Testisin/Prss21 Antibody [NBP1-84789] - Staining of human prostate shows no membranous positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Testisin/Prss21 Antibody [NBP1-84789] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.

Product Discontinued

Testisin/Prss21 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CFETYSDLSDPSGWMVQFGQLTSMPSFWSLQAYYTRYFVSNIYLSPRYLGNSPYDIALVKLSAPVTYTKHIQPICLQASTFEFENRTDCWVTGWGYIKEDEALPSPHTL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRSS21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Testisin/Prss21 Antibody

• EC 3.4.21
• Eosinophil serine protease 1
• ESP1EC 3.4.21.-
• ESP-1serine protease from eosinophils
• protease, serine, 21 (testisin)
• Prss21
• Serine protease 21
• TEST1
• TEST1testisin
• Testisin

Background

Testisin encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. It is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and it may be involved in progression of testicular tumors of germ cell origin. Alternative splicing of this gene results in three transcript variants encoding three different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRSS21