TDAG8/GPR65 Overexpression Lysate (Native)

Images

 
Western Blot: GPR65 Overexpression Lysate (Adult Normal) [NBP2-04251] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR65.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TDAG8/GPR65 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_003599. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens GPR65, mRNA.
Gene
GPR65

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TDAG8/GPR65

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TDAG8/GPR65 Overexpression Lysate (Native)

  • G protein-coupled receptor 65
  • GPR65
  • G-protein coupled receptor 65
  • Psychosine Receptor
  • T-cell death-associated gene 8 protein
  • TDAG8
  • TDAG8hTDAG8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TDAG8/GPR65 Lysate (NBP2-04251). (Showing 1 - 1 of 1 FAQ).

  1. Has the anti-GPR65 antibody, NBP2-24487, been tested in the overexpressing lysate, NBP2-04251?
    • We do not have data from the tests if indeed these have been used together, but it would make sense to utilize the lysate as a positive control for your western blot if needed.

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Bioinformatics

Gene Symbol GPR65
Entrez