TCP1-eta Antibody (PK/16/8/a)

Images

 
Western Blot: TCP1-eta Antibody (PK/16/8/a) [NB100-66571] - Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with RAT ANTI CCT ETA followed by F(ab')2 RABBIT ANTI ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clone
PK/16/8/a
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TCP1-eta Antibody (PK/16/8/a) Summary

Immunogen
Made to CCT ETA
Specificity
NB100-66571 recognizes the eta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCT7
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. In WB detects a band of approximately 60kD in Hela cell lysates.

Reactivity Notes

Cross reacts with Mouse, Human, Bovine and Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TCP1-eta Antibody (PK/16/8/a)

  • CCTETA
  • CCT-eta
  • CCTH
  • chaperonin containing TCP1, subunit 7 (eta)
  • eta subunit
  • HIV-1 Nef interacting protein
  • HIV-1 Nef-interacting protein
  • MGC110985
  • Nip7-1
  • T-complex protein 1 subunit eta

Background

This antibody recognizes the eta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring structure. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP dependent manner. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumor suppressor protein. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCT7
Entrez
Uniprot