TCP1 alpha Antibody (91a)

Western Blot: TCP1 alpha Antibody (91a) [NBP2-59347] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a (NBP2-59347). Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody (NBP2-59347) at 1:1000.

Product Discontinued

TCP1 alpha Antibody (91a) Summary

• Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
• Localization: Cytoplasm
• Specificity: Detects 60kDa. Also detects 92kDa. Cross reactivity with human HSP60 has been observed with this in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TCP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS pH7.4, 50% glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for TCP1 alpha Antibody (91a)

• CCT1T-complex protein 1 subunit alpha
• CCTa
• CCT-alpha
• D6S230E
• tailless complex polypeptide 1
• t-complex 1
• T-complex protein 1, alpha subunit
• TCP-1-alpha

Background

TCP1 alpha encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCP1