TCP1 alpha Antibody (23c)

Images

 
Western Blot: TCP1 alpha Antibody (23c) [NB120-13501] Lane 1: MW marker, Lane 2: Hsp60 Recombinant Human Protein, Lane 3: 3T3 Heat Shocked, Lane 4: PC-12 Heat Shocked.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Ha, Pl, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
23c
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TCP1 alpha Antibody (23c) Summary

Immunogen
Recombinant mouse TCP-1 alpha (C-terminal fragment).
Epitope
Ab13501 recognises the COOH group, addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
Localization
Cytoplasmic
Specificity
This recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human Hsp60 protein.
Isotype
IgG2c
Clonality
Monoclonal
Host
Rat
Gene
TCP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2-5 ug/ml
Application Notes
Detects TCP1 alpha in 20 ug of RK13 heat shocked cell lysate. Predicited molecular weight: 60 kDa. Also detects a molecular mass band of approximately 92 kDa.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 50% glycerol.
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TCP1 alpha Antibody (23c)

  • CCT1T-complex protein 1 subunit alpha
  • CCTa
  • CCT-alpha
  • D6S230E
  • tailless complex polypeptide 1
  • t-complex 1
  • T-complex protein 1, alpha subunit
  • TCP-1-alpha

Background

T complex polypeptide 1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TCP1
Entrez