TCP1 alpha Antibody (23c (CTA-123)) [CoraFluor™ 1]

• Reactivity: Mu, Rt, Ca, Ch, Ha
• Applications: WB, ICC/IF, IHC, IP
• Clone: 23c (CTA-123)
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

TCP1 alpha Antibody (23c (CTA-123)) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: C-terminal half of the full length murine TCP protein
• Specificity: Does not recognize primate, xenopus or yeast TCP-1.
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Rat
• Gene: TCP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TCP1 alpha Antibody (23c (CTA-123)) [CoraFluor™ 1]

• CCT1T-complex protein 1 subunit alpha
• CCTa
• CCT-alpha
• D6S230E
• tailless complex polypeptide 1
• t-complex 1
• T-complex protein 1, alpha subunit
• TCP-1-alpha

Background

TCP1 alpha encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCP1