TAS2R4 Antibody

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

TAS2R4 Antibody Summary

• Immunogen: A synthetic peptide from human TAS2R4 conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAS2R4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: Useful in Immunofluorescence (PMID: 21303656).

Reactivity Notes

Marmoset

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TAS2R4 Antibody

• candidate taste receptor T2R4
• taste receptor, type 2, member 4

Background

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. FUNCTION: Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. MISCELLANEOUS: Several bitter taste receptors are expressed in a single taste receptor cell. Useful Links; The Human Olfactory Data Exploratorium: HORDE; Olfactory Receptor Data Base: ORDB; Transmembrane Analysis: TM analysis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAS2R4 Antibody (NB110-74890)(2)

Publications using NB110-74890

• Yan CH, Hahn S, McMahon D et al. Nitric oxide production is stimulated by bitter taste receptors ubiquitously expressed in the sinonasal cavity Am J Rhinol Allergy. 2017-02-28 [PMID: 28452704] (ICC/IF, Human)
• Singh N, Vrontakis M, Parkinson F, Chelikani P. Functional bitter taste receptors are expressed in brain cells. Biochem Biophys Res Commun;406(1):146-51. 2011-03-04 [PMID: 21303656]

Bioinformatics

• Gene Symbol: TAS2R4
• Entrez:
  • 50832(Human)
• Uniprot:
  • Q9NYW5(Human)