Tankyrase binding protein 1 Antibody

Immunohistochemistry-Paraffin: Tankyrase binding protein 1 Antibody [NB300-988] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tankyrase binding protein 1 Antibody Summary

• Immunogen: Peptide with sequence KVSTLRESSAMAS-C corresponding to N-Terminus according to NP_203754.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNKS1BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments in human ovary and lung lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tankyrase binding protein 1 Antibody

• KIAA1741TAB182FLJ45975,182 kDa tankyrase-1-binding protein
• tankyrase 1 binding protein 1, 182kDa
• tankyrase 1-binding protein of 182 kDa

Background

TAB182 (tankyrase 1-binding protein of 182 kDa) was identified in a two-hybrid screen in search of tankyrase 1-interacting proteins. Tankyrase 1 is a poly (ADP-ribose) polymerase (PARP) that ribosylates the negative regulator of telomere length, TRF1 (telomere-repeat binding factor). Ribosylation of TRF1 has been shown to result in the inhibition of TRF1 binding and an induction of telomere elongation. TAB182 and TRF1 bind multiple discrete and overlapping sites of the tankyrase 1 ankyrin domain. This finding suggests that TAB182 may act as a scaffold to mediate higher order protein complex formation at telomeres. TAB182 has also been suggested to serve a role in the cytoplasm as it has been found to localize to the cytoplasmic cortical actin network.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tankyrase binding protein 1 Antibody (NB300-988)(1)

Publication using NB300-988

• Seimiya H, Smith S. The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182). J Biol Chem 2002-04-19 [PMID: 11854288]

Bioinformatics

• Gene Symbol: TNKS1BP1
• Entrez:
  • 85456(Human)
• Uniprot:
  • NP_203754.(Human)