TAFA5/FAM19A5 Antibody [Unconjugated]

Western blot shows lysates of IMR‑32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TAFA5/FAM19A5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 10.

Product Discontinued

TAFA5/FAM19A5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TAFA5/FAM19A5
  Gln26-Ser125
  Accession # NP_056196
• Specificity: Detects human and mouse TAFA5/FAM19A5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FAM19A5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAFA5/FAM19A5 Antibody [Unconjugated]

• Chemokine-like protein TAFA-5
• FAM19A5
• family with sequence similarity 19 (chemokine (C-C motif)-like), member A5
• QLLK5208
• TAFA protein 5
• TAFA5
• TAFA-5
• UNQ5208

Background

TAFA5 (also FAM19A5) is a 14 kDa type I transmembrane protein and member of the FAM19/TAFA family of chemokine-like proteins (1). Human TAFA5 is 132 amino acids (aa) in length. It contains a 15 aa extracellular domain, a 23 aa transmembrane sequence, and a 95 aa cytoplasmic region. Alternate splicing produces two additional isoforms. Isoform 2, a secreted form, has a 31 aa substitution for residues 1‑38 in isoform 1. Isoform 3 has an eight aa substitution for residues 1‑87 in isoform1. Human TAFA5 is 100% aa identical to mouse TAFA5 (1). Within the TAFA family, TAFA5 is the most distinct member, while TAFAs 2, 3, and 4 are the most closely related members (1). Real-time PCR analysis indicates that TAFA5 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the basal ganglia and cerebellum (1). The biological functions of TAFA family members are not yet known, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1‑2). Finally, TAFAs may control axonal sprouting following brain injury (1).

1. Tang, Y.T. et al. (2004) Genomics 83:727.
2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAM19A5
• Uniprot:
  • NP_056196()