TAFA3/FAM19A3 Antibody (460904)

A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA3/FAM19A3 Monoclonal Antibody (Catalog # MAB4677, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 460904
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TAFA3/FAM19A3 Antibody (460904) Summary

• Immunogen: E. coli-derived recombinant human TAFA3/FAM19A3
  Ala31-Arg133
  Accession # Q7Z5A8
• Specificity: Detects human TAFA3/FAM19A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 4, or 5 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAM19A3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAFA3/FAM19A3 Antibody (460904)

• Chemokine-like protein TAFA-3
• FAM19A3
• family with sequence similarity 19 (chemokine (C-C motif)-like), member A3
• MGC138473
• TAFA3
• TAFA-3

Background

TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha , sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAM19A3
• Uniprot:
  • Q7Z5A8(Human)