TAF3B1 Antibody

Product Discontinued

TAF3B1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human TFIIIB90-1.
• Specificity: BDP1 (E257) detects endogenous levels of BDP1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of TFIIIB90-1 (E257) pAb in extracts from HeLa cells .
  Immunohistochemistry (IHC) analyzes of TFIIIB90-1 (E257) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TAF3B1 Antibody

• B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
• DKFZp686K0831
• RNA polymerase III, GTF3B subunit 1
• TAF3B1
• TFIIIB150
• TFIIIB90
• TFNR
• transcription factor-like nuclear regulator

Background

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TAF3B1 Antibody (NBP1-01035). (Showing 1 - 1 of 1 FAQ).

1. I am wondering why the shown Western Blot for the antibody for BDP1 (NBP1-01035) shows only one single band, since this protein is described to have eight different isoforms. Does this antibody detect only one of them?
   • NBP1-01035 has been shown to detect isoform 1 or the canonical sequence of BDP1. Although you are correct that this protein has 8 potential isoforms as reported in uniprot, isoforms 5-8 lack experimental confimation. We have not tested the HeLa cell extracts and our antibody to determine if our antibody specifically recognizes only isoform 1 of the protein or if in fact the other isoforms are simply not abundantly expressed.

Bioinformatics

• Gene Symbol: BDP1
• Entrez:
  • 55814(Human)