Syntaxin 5 Antibody

Western Blot: Syntaxin 5 Antibody [NBP3-06282] - Anti-STX5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:40 kDaObserved band size:42 kDa(We are unsure as to the identity of these extra bands.)

Immunoprecipitation: Syntaxin 5 Antibody [NBP3-06282] - STX5 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-STX5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-STX5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :43 kDa

Product Discontinued

Syntaxin 5 Antibody Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Syntaxin 5 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STX5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Syntaxin 5 Antibody

• SED5
• STX5
• STX5ASED5
• Syntaxin 5
• syntaxin 5A
• syntaxin-5

Background

Syntaxin 5 mediates endoplasmic reticulum to Golgi transport . Golgi apparatus membrane; Single-pass type IV membrane protein (Potential)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX5