STRA6 Antibody (496613) [Unconjugated]

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." > MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." title="HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." />

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904, filled histogram) or isotype control antibody (Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." > NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." title="STRA6 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." />

STRA6 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 496613
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STRA6 Antibody (496613) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human STRA6
  Met1-Pro667
  Accession # AAH25256
• Specificity: Detects human STRA6 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STRA6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STRA6 Antibody (496613) [Unconjugated]

• FLJ12541
• MCOPS9
• stimulated by retinoic acid gene 6 homolog (mouse)
• stimulated by retinoic acid gene 6 protein homolog
• STRA6

Background

Stimulated by retinoic acid gene 6 protein homolog (STRA6) is a 74 kDa broadly expressed transmembrane protein that does not belong to any particular family of proteins. Human STRA6 is 667 amino acids (aa) in length. It contains nine transmembrane regions and one potential N-linked glycosylation site in the first extracellular domain. In addition, there are three splicing variants producing four isoforms. There is no signal sequence. Human STRA6 is 80% identical to bovine STRA6 and 74% identical to mouse and rat STRA6. STRA6 may act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Defects in STRA6 are the cause of Spear syndrome and Matthew-Wood syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STRA6 Antibody (MAB5904)(1)

Publication using MAB5904

• Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H; Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells Cells 2023-08-19 [PMID: 37626914] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: STRA6
• Uniprot:
  • AAH25256()