STK38 Antibody

Western Blot: STK38 Antibody [NBP2-27552] - NBP2-27552 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STK38 Antibody Summary

• Immunogen: Peptide with sequence C-KPTVATSNHPET corresponding to C-Terminus (near) according to NP_009202.1.
• Specificity: This antibody is NOT expected to cross-react with STK38L (GeneID 23012).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STK38
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human and rat spleen lysates (calculated MW of 54.2 kDa band according to NP_009202.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STK38 Antibody

• EC 2.7.11
• EC 2.7.11.1
• Ndr Ser/Thr kinase-like protein
• NDR1 protein kinase
• NDR1
• NDRnuclear Dbf2-related 1
• Nuclear Dbf2-related kinase 1
• serine threonine protein kinase
• serine/threonine kinase 38
• serine/threonine-protein kinase 38

Background

STK38 belongs to the NDR family of serine/threonine protein kinases. NDR kinases require the phosphorylation of conserved Ser/Thr residues for activation. NDR family members have two unique stretches of primary sequence: an N-terminal regulatory (NTR) domain and an insert of several residues between subdomains VII and VIII of the kinase domain. The kinase domain insert functions as an auto-inhibitory sequence (AIS), while binding of the co-activator MOB (Mps-one binder) proteins to the NTR domain releases NDR kinases from inhibition of autophosphorylation. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STK38 Antibody (NBP2-27552)(1)

Publication using NBP2-27552

• Kohler RS, Schmitz D, Cornils H et al. Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases. Mol Cell Biol 2010-09-01 [PMID: 20624913]

Bioinformatics

• Gene Symbol: STK38
• Entrez:
  • 361813(Rat)
• Uniprot:
  • NP_009202.1(Human)