SR140 Antibody (983615) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse SR140 Monoclonal Antibody (Catalog # MAB9897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR140 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 983615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SR140 Antibody (983615) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human SR140 Peptide
  Accession # O15042
• Specificity: Detects human SR140 in direct ELISAs. Detects human and mouse SR140 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SR140 Antibody (983615) [Unconjugated]

• 140 kDa Ser/Arg-rich domain protein
• FSAPa
• KIAA0332
• SR140
• U2 snRNP-associated SURP domain containing
• U2 snRNP-associated SURP motif-containing protein
• U2-associated protein SR140
• U2-Associated SR140 Protein
• U2SURP

Background

SR140, also known as U2-associated protein SR140 (U2SURP) is a 118 kDa protein located in the nucleus. SR140 belongs to the splicing factor SR family and has 3 known isoforms that are produced by alternative splicing. The protein amino acid sequence is highly conserved across species and can be heavily phosphorylated, leading to a shift in observed molecular weight.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O15042()