Spinesin Antibody

Western Blot: Spinesin Antibody [NB100-896] - Staining of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Spinesin Antibody Summary

• Description: Novus Biologicals Goat Spinesin Antibody (NB100-896) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LDWIHDTAQDSLL corresponding to C-Terminus according to NP_110397.2.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMPRSS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 50 kDa band and 29 kDa band in human brain is reported in scientific literature (PMID: 11741986). This protein has a calculated MW of 49.6 kDa band according to NP_110397.1. IHC: Paraffin embedded Human Liver and Small Intestine.
• Control:

  Spinesin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Spinesin Antibody

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• MGC141886
• MGC148044
• Spinesin
• TMPRSS5
• transmembrane protease serine 5
• transmembrane protease, serine 5

Background

Spinesin is a protein that belongs to the serine protease family which has been known to be involved in many physiological and pathological processes. Spinesin has been shown to be expressed in neurons, in their axons, and at the synapses of motoneurons in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMPRSS5
• Entrez:
  • 80975(Human)
  • 80893(Mouse)
  • 266681(Rat)
• Uniprot:
  • NP_110397()