Sodium-Calcium Exchanger Antibody (C2C12)

Images

 
Immunocytochemistry/ Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. ...read more
Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.
Immunocytochemistry/ Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. ...read more
Immunocytochemistry/ Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. ...read more
Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Gp, I, Ma, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
C2C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Sodium-Calcium Exchanger Antibody (C2C12) Summary

Immunogen
Purified canine cardiac sodium/calcium exchanger.
Epitope
This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.
Specificity
Sodium / Calcium Exchanger (C2C12)
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
SLC8A3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
Application Notes
ELISA usage was reported in the scientific literature (PMID: 7692739). WB: Detects an approx. 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. Also detects 70 kDa and 160 kDa proteins representing a proteolytic fragment and non-reduced exchanger respectively. T-tubular membrane.
Publications
Read Publications using
NB120-2869 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS +BSA(1mg/ml)
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Sodium-Calcium Exchanger Antibody (C2C12)

  • Na(+)/Ca(2+)-exchange protein 3
  • Na+/Ca2+ exchanger 3
  • NCX3sodium/calcium exchanger SLC8A3
  • sodium/calcium exchanger 3
  • solute carrier family 8 (sodium/calcium exchanger), member 3
  • solute carrier family 8 (sodium-calcium exchanger), member 3

Background

The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sodium-Calcium Exchanger Antibody (NB120-2869)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Human
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SLC8A3