SNX26 Antibody

Immunohistochemistry-Paraffin: SNX26 Antibody [NB100-53781] - Stainingof paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SNX26 Antibody Summary

• Immunogen: Peptide with sequence ARSTDSLDGPGE-C corresponding to N-Terminus according to NP_443180.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGAP33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1.25 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 35 kDa band in human brain (hippocampus) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 121 kDa band according to NP_443180.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows cytoplasm staining of secretory cells in the glands.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX26 Antibody

• FLJ39019
• neurite outgrowth multiadaptor RhoGAP protein
• NOMA-GAP
• Rho GTPase activating protein 33
• rho GTPase-activating protein 33
• Rho-type GTPase-activating protein 33
• sorting nexin 26
• Sorting nexin-26
• Tc10/CDC42 GTPase-activating protein
• TCGAPSNX26

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. The specific function of this protein has not been elucidated. Alternative splice variants have been described but their full length nature has not been determined. SNX26 may be involved in several stages of intracellular trafficking. It could play an important role in the regulation of glucose transport by insulin. It may act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX26 Antibody (NB100-53781)(1)

Publication using NB100-53781

• Liu H, Nakazawa T, Tezuka T, Yamamoto T. Physical and functional interaction of FYN tyrosine kinase with a brain-enriched RHO GTPASE-activating protein TCGAP. J Biol Chem 2006-06-15 [PMID: 16777849]

Bioinformatics

• Gene Symbol: ARHGAP33
• Entrez:
  • 115703(Human)
• Uniprot:
  • NP_443180.2(Human)