SMG1 Antibody (1C12) - Azide and BSA Free

Western Blot: SMG1 Antibody (1C12) [H00023049-M03] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).

Immunohistochemistry-Paraffin: SMG1 Antibody (1C12) [H00023049-M03] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml

ELISA: SMG1 Antibody (1C12) [H00023049-M03] - Detection limit for recombinant GST tagged SMG1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SMG1 Antibody (1C12) - Azide and BSA Free Summary

• Immunogen: SMG1 (NP_055535, 2922 a.a. ~ 3031 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV
• Specificity: SMG1 (1C12)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMG1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SMG1 Antibody (1C12) - Azide and BSA Free

• ATXserine/threonine-protein kinase SMG1
• EC 2.7.11
• EC 2.7.11.1
• hSMG-1,61E3.4
• KIAA0421phosphatidylinositol 3-kinase-related protein kinase
• Lambda/iota protein kinase C-interacting protein
• Lambda-interacting protein
• LIPPI-3-kinase-related kinase SMG-1
• phosphatidylinositol 3-kinase-related kinase
• SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
• SMG-1

Background

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMG1
• Entrez:
  • 23049(Human)
• OMIM:
  • 607032(Human)
• Uniprot:
  • NP_054725.1(Human)