SLITRK6 Antibody (753826) [Unconjugated]

SLITRK6 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human SLITRK6 Monoclonal Antibody (Catalog # MAB7317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 753826
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SLITRK6 Antibody (753826) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SLITRK-6
  Ile16-Ala604
  Accession # Q9H5Y7
• Specificity: Detects human SLITRK-6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLITRK6 Antibody (753826) [Unconjugated]

• FLJ22774
• MGC119595
• MGC119596
• MGC119597,4832410J21Rik
• SLIT and NTRK-like family, member 6
• SLIT and NTRK-like protein 6
• slit and trk like gene 6
• SLITRK6

Background

SLITRK6 (Slit and NTRK-like 6) is a 95 kDa (predicted) member of the SLITRK family of proteins. It is widely expressed in the embryo, including cells in limb bud mesenchyme, visceral organ primodia, cells of the dorsal thalamus, and cochlear plus vestibular sensory epithelium. In the ear, SLITRK6 is suggested to induce neurotrophin expression from cells, thus promoting sensory neuron survival and axon pathfinding. Mature human SLITRK6 is an 826 amino acid (aa) type I transmembrane protein. It contains a 593 aa extracellular domain (ECD) (aa 16-608) plus a 212 aa cytoplasmic region. The ECD possesses two LRR clusters, one that involves seven LRRs (aa 27-269), and a second that involves eight LRRs (aa 320-568). Over aa 16-604, human SLITRK6 shares 89% aa sequence identity with mouse SLITRK6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9H5Y7(Human)