SLC34A1 Antibody (10B1.3E9) - BSA Free

Western Blot: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - WB detection of SLC34A1 /NPTIIa protein in a lysate of mouse thymus using SLC34A1 clone 10B1.3E9 at its 1:500 dilution. The antibody detected a single specific band at ~80 kDa representing the glycosylated form of SLC34A1 protein.

Immunocytochemistry/Immunofluorescence: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized using 1X TBS + 0.5% Triton-X100. The cells were incubated with hSLC34A1 (10B1.3E9) at a 1:50 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected with Phalloidin 568 (Red). Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - IHC analysis of a formalin-fixed and paraffin-embedded tissue section of human normal kidney using SLC34A1 antibody (clone 10B1.3E9) at 1:75 dilution. The epithelial cells of various renal ducts and tubules depicted very nice membrane-cytoplasmic SLC34A1 immunostaining while the Bowman's capsule and the nuclei of cells were largely negative for SLC34A1 protein.

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - FLOW detection of SLC34A1 protein on HEK293 cells - After fixation and permeabilization, 2 x 10^6 cells/ml were stained using SLC34A1 antibody (clone 10B1.3E9) at 1:1000 dilution. Signal was developped using GtxMs dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 10B1.3E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SLC34A1 Antibody (10B1.3E9) - BSA Free Summary

• Immunogen: Partial synthetic peptide made to an internal portion of the human SLC34A1 protein (between amino acids 25-150) [UniProt Q06495]
• Localization: Membrane-Cytoplasmic
• Marker: Tubulointerstitium Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC34A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation reported in scientific literature (PMID 35307350)
  • Western Blot 1:500
• Application Notes: SLC34A1 is a 639 amino acids long protein with predicted molecular weight of 68.9 kDa, however, because of glycosylation, the processed protein may show up at higher than predicted molecular weight position on Western blot.
• Theoretical MW: 68.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SLC34A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SLC34A1 Antibody (10B1.3E9) - BSA Free

• FRTS2
• Na(+)/Pi cotransporter 2A
• Na(+)-dependent phosphate cotransporter 2A
• naPi-2a
• NAPI-3
• NPT2NPHLOP1
• NPTIIa
• renal sodium-dependent phosphate transporter
• SLC11
• SLC17A2Na+-phosphate cotransporter type II
• Sodium/phosphate cotransporter 2A
• sodium/phosphate co-transporter
• sodium-dependent phosphate transport protein 2A
• Sodium-phosphate transport protein 2A
• solute carrier family 17 (sodium phosphate), member 2
• solute carrier family 34 (sodium phosphate), member 1
• Solute carrier family 34 member 1

Background

SLC34A1 / NaPi-IIa [Solute carrier family 34 (type II sodium/phosphate cotransporter), member 1] belongs to the type II sodium-phosphate cotransporter family and is a multi-pass membrane protein critically involved in the maintenance of renal inorganic phosphate (Pi) homeostasis and the plasma Pi levels. SLC34A1 acts as the most significant regulator of Pi homeostasis through its reabsorption via Na(+) cotransport in the brush border membrane of renal tissues. It is mainly expressed in the kidney wherein it accounts for 70-80% of the apical influx, however, its presence in some other tissues such as lungs, testes, fallopian tubes, and heart myocytes has also been documented (UniProt and Human Protein Atlas project databases). Signaling implicated in SLC34A1's regulation include the protein kinases A /C, ERK1/2, Klotho/KL, the PI3K/PKB/GSK3 kinase cascade, NHERF1/ SLC9A3R1, AMP-activated protein kinase AMPK, JAK2, and B-RAF. Moreover, SLC34A1 expression has been reported to be controlled by dietary Pi and hormones namely 1,25-dihydroxyvitamin D3, FGF23, Dopamine, Thyroid Hormone, Glucocorticoids, Estrogen /E2 and Triiodothyronine/T3. Besides renal Pi homeostasis, SLC34A1 has been suggested to be involved in several biological processes such as kidney development, bone remodeling, dentinogenesis, cholesterol metabolism, cellular response to metal ion/drug, parathyroid hormone stimulus, protein homooligomerization etc. SLC34A1 mutations have been shown to be associated with diseases such as Nephrolithiasis/osteoporosis, hypophosphatemic, 1 (NPHLOP1), Fanconi renotubular syndrome 2 (FRTS2) and idiopathic infantile hypercalcemia (IIH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC34A1 Antibody (NBP2-42216)(5)

Publications using NBP2-42216

• Friedman PA, Sneddon WB, Mamonova T et al. RGS14 regulates hormone-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein The Journal of biological chemistry 2022-03-17 [PMID: 35307350] (IP, Human)
• Qian J, Zhong J, Liu S Et Al. alpha-Klotho, Plasma Asymmetrical Dimethylarginine, and Kidney Disease Progression Kidney Med 2021-12-23 [PMID: 34939007]
• Bayer J, Vaghela R, Drechsler S et al. The bone is the major source of high circulating intact fibroblast growth factor-23 in acute murine polymicrobial sepsis induced by cecum ligation puncture PloS one 2021-05-14 [PMID: 33989306] (WB, Mouse)
• Dhillon P, Park J, Hurtado Del Pozo C et al. The Nuclear Receptor ESRRA Protects from Kidney Disease by Coupling Metabolism and Differentiation Cell metabolism 2020-12-01 [PMID: 33301705] (IF/IHC)
• Martins JS, Liu ES, Sneddon WB, et al 1,25-dihydroxyvitamin D maintains brush border membrane NaPi2a and attenuates phosphaturia in Hyp mice Endocrinology 2019-06-25 [PMID: 31237611]

Review for SLC34A1 Antibody (NBP2-42216) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 08/17/2021

Summary

• Application: Western Blot
• Sample Tested: Kidney tissue
• Species: Mouse
• Lot: 10B1.3E9

Bioinformatics

• Gene Symbol: SLC34A1
• Entrez:
  • 6569(Human)
• Uniprot:
  • Q06495(Human)