SLC34A1 Overexpression Lysate

Images

 
Western Blot: SLC34A1 Overexpression Lysate (Adult Normal) [NBP2-08042] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC34A1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Host
Mouse

Order Details

SLC34A1 Overexpression Lysate Summary

Description
Transient overexpression lysate of solute carrier family 34 (sodium phosphate), member 1 (SLC34A1), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC208782 and based on accession number NM_003052. The protein contains a C-MYC/DDK Tag.
Localization
Membrane-Cytoplasmic
Marker
Tubulointerstitium Marker
Host
Mouse
Gene
SLC34A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The immunogen has 78% sequence homology to Rat species.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC34A1 Overexpression Lysate

  • FRTS2
  • Na(+)/Pi cotransporter 2A
  • Na(+)-dependent phosphate cotransporter 2A
  • naPi-2a
  • NAPI-3
  • NPT2NPHLOP1
  • NPTIIa
  • renal sodium-dependent phosphate transporter
  • SLC11
  • SLC17A2Na+-phosphate cotransporter type II
  • Sodium/phosphate cotransporter 2A
  • sodium/phosphate co-transporter
  • sodium-dependent phosphate transport protein 2A
  • Sodium-phosphate transport protein 2A
  • solute carrier family 17 (sodium phosphate), member 2
  • solute carrier family 34 (sodium phosphate), member 1
  • Solute carrier family 34 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SLC34A1 Lysate (NBP2-08042) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SLC34A1 Lysate (NBP2-08042). (Showing 1 - 1 of 1 FAQ).

  1. What antibody did you use for Western blot of this SLC34A1 overexpressing Lysate (NBP2-08042)?
    • We do not currently sell a SLC34A1 antibody that has been validated in Western blot. The image for NBP2-08042 was generated using a high-throughput screening using an antibody to the C-terminal DDK-tag (NBP1-06712).

Other Available Formats

NBP2-08042

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Blogs on SLC34A1.

SLC34A1 - major regulator of inorganic phosphate (Pi) homeostasis
SLC34A1 encodes the 69 kDa sodium-dependent phosphate transport protein 2A (Npt2a). SLC34A is a member of the type II sodium-phosphate co-transporter family, along with SLC34A3 which encodes Npt2c. These proteins are abundantly expressed along the...  Read full blog post.

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Bioinformatics

Gene Symbol SLC34A1