SLC30A7 Knockout HeLa Cell Lysate

Images

 

Product Details

Summary
Product Discontinued
View other related SLC30A7 Lysates

Order Details


    • Catalog Number
      NBP2-65312
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

SLC30A7 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,4 bp deletion in exon 1 and 1 bp insertion in exon 1
Gene
SLC30A7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SLC30A7 Knockout HeLa Cell Lysate

  • DKFZp686M0368
  • solute carrier family 30 (zinc transporter), member 7
  • Solute carrier family 30 member 7
  • zinc transporter like 2
  • zinc transporter ZnT-7
  • ZnT-7
  • ZNT7zinc transporter 7
  • ZnTL2
  • Znt-like transporter 2

Background

Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc (Seve et al., 2004 [PubMed 15154973]).[supplied by OMIM, Mar 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-86825
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NBP3-41516
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-31673
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-86824
Species: Hu
Applications: IHC,  IHC-P
NBP3-41280
Species: Hu
Applications: WB
NBP2-06015
Species: Hu
Applications: WB
NBP2-31825
Species: Hu
Applications: IHC,  IHC-P
AF7315
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
NBP1-76498
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC,  IHC-P, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr,  IHC-P, IP, WB
NBP1-49643
Species: Bv, Hu, Pm
Applications: Flow-IC, Flow, IB, ICC/IF, IHC,  IHC-P, KO, WB
NBP1-76510
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC,  IHC-P, WB
MAB6095
Species: Hu
Applications: KO, WB
NBP1-85540
Species: Hu
Applications: IHC,  IHC-P, WB
NBP1-86380
Species: Bv, Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr,  IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
DPI00
Species: Hu
Applications: ELISA
NBP1-76507
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC,  IHC-P, WB

Publications for SLC30A7 Lysate (NBP2-65312) (0)

There are no publications for SLC30A7 Lysate (NBP2-65312).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SLC30A7 Lysate (NBP2-65312) (0)

There are no reviews for SLC30A7 Lysate (NBP2-65312). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SLC30A7 Lysate (NBP2-65312) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SLC30A7 Products

Research Areas for SLC30A7 Lysate (NBP2-65312)

Find related products by research area.

Blogs on SLC30A7

There are no specific blogs for SLC30A7, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SLC30A7 Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC30A7