Immunohistochemistry-Paraffin: SLC2A7 Antibody [NBP1-81821] - Staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.
Simple Western: SLC2A7 Antibody [NBP1-81821] - Simple Western analysis of SLC2A7 in TC7 cells using SLC2A7 antibody. Cells were untreated or treated with Rapid PNGase F. Note: Deglycosylation of the protein is necessary ...read more
1:10 in Antibody diluent (ProteinSImple), 30 min RT,
Goat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#
Chemiluminescence (luminol/peroxide), multiimage analysis (5s-480s). Shift from ~58 kDa to ~47kDa peak after deglycosylation.
Since the antibody is targeting glycosylated epitope, it is essential to deglycosylate the protein to enhance the detection in Simple Western. Gluts are generally prone to aggregation and so the deglycosylation (15 min) as well as denaturation (10 min) were performed at 37oC.
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