SIRPB2 Antibody (1008702) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) SIRP beta 2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

THP-1 monocytic leukemia cell line was stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981, filled histogram) or Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1008702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SIRPB2 Antibody (1008702) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line, HEK293 derived Human SIRP beta 2
  Gln33-Gly287
  Accession # Q5JXA9
• Specificity: Detects human SIRP beta 2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRPB2 Antibody (1008702) [Unconjugated]

• dJ776F14.2
• Protein Tyrosine Phosphatase Non-Receptor Type Substrate 1-Like 3
• Protein Tyrosine Phosphatase Non-Receptor Type Substrate Protein
• Protein Tyrosine Phosphatase, Non-Receptor Type 1-Like
• Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1-Like 3
• PTPN1L
• PTPNS1L3
• Signal-Regulatory Protein Beta 2
• Signal-Regulatory Protein Beta-2
• SIRP beta 2
• SIRP-beta-2
• SIRPG

Background

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

1. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
2. Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
3. Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
4. Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
5. Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
6. Veillette A. (2018) Trends Immunol. 39:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q5JXA9()