SGK3 Overexpression Lysate (Native)

Images

 
Western Blot: SGK3 Overexpression Lysate (Adult Normal) [NBL1-15909] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SGK3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SGK3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214835 and based on accession number NM_001033578. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 3, mRNA.
Gene
SGK3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
56.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SGK3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SGK3 Overexpression Lysate (Native)

  • CISK
  • DKFZp781N0293
  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine-protein kinase Sgk3
  • serum/glucocorticoid regulated kinase 3
  • serum/glucocorticoid regulated kinase family, member 3
  • serum/glucocorticoid regulated kinase-like
  • Serum/glucocorticoid-regulated kinase 3
  • Serum/glucocorticoid-regulated kinase-like
  • SGK2
  • SGKLcytokine-independent survival kinase

Background

This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SGK3 Lysate (NBL1-15909) (0)

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Bioinformatics

Gene Symbol SGK3