sFRP-1 Antibody

Western Blot: sFRP-1 Antibody [NBP2-89148] - Analysis of SFRP1 on SH-SY-5Y cells lysates using anti-SFRP1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: sFRP-1 Antibody [NBP2-89148] - Staining SFRP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP2-89148] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-sFRP-1 antibody. Counter stained with hematoxylin.

Flow Cytometry: sFRP-1 Antibody [NBP2-89148] - Analysis of SH-SY-5Y cells with SFRP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: sFRP-1 Antibody [NBP2-89148] - Staining SFRP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP2-89148] - Analysis of paraffin-embedded rat testis tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP2-89148] - Analysis of paraffin-embedded human colon cancer tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP2-89148] - Analysis of paraffin-embedded human placenta tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP2-89148] - Analysis of paraffin-embedded mouse small intestine tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.

Product Discontinued

sFRP-1 Antibody Summary

• Immunogen: Synthetic peptide within N-terminal human sFRP-1. (SwissProt: Q8N474 Human; SwissProt: Q8C4U3 Mouse; SwissProt: Q9R168 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFRP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for sFRP-1 Antibody

• FRP1
• FRP-1FrzA
• FRPSARP-2
• FrzA
• SARP-2
• SARP2sFRP-1
• Secreted apoptosis-related protein 2
• secreted frizzled-related protein 1
• sFRP1
• sFRP-1

Background

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFRP1